4. Example: Mycetophila uninotata

Before starting dissection we take a photo of the whole body, to show coloration and size ratio. We hold the sixth segment, the last "normal" one, with Dumont forceps and cut off the terminalia right before it.

After clearing, we insert a blunt pin from the left (left photo below), press it sideways to the bottom, pierce the acute scalpel into the mebrane right before the ninth segment, and pull untile we can see the reduced 7th and 8th segments. The only sclerotized link of the ninth segment to the rest is a small ventral fork. Try to separate the ninth segment by piercing with the scalpel exactly between fork and the ninth segment. Remove the membranous dorsal connection. Now we take a caudal view to get a first glimpse inside, with the dorsal lobe of the gonstylus above and ventral one below.

In the dorsal view on the left below we see the small ninth tergite (left) with the cerci, both obstructing the view on the aedeagus. The problem is that the middle part of the ninth tergite is often weaker, than the membraneous connection to the right and left gonocoxites. So we insert the blunt pin into the anterior side (from the left) between aedeagus and gonocoxa and bend away the cerci. Then we crack the membrane on both sides and pull the ninth tergite away.

Inserting the blunt pin again between aedeagus and gonocoxite, we press down the hypopygium to the bottom and carefully bend out the right gonostylus. This is the only place where I use the word "careful" in the examples, because you must watch out where you can touch the gonostylus without loosing bristles. In the left photo below we see the left ventral lobe in front and the whole bent out gonostylus behing. Now find a place between right gonostylus and gonocoxa, where you can pierce in the acute scalpel. Repeat that several times until you can pull it away. Now we could embed the gonostylus for better resolution, but the natural orientation between dorsal and ventral lobe is quite unlucky, that we better separate them. So we use the highest magnification of the binocular, orient the gonostylus with the scalpel and pierce it down on the joint between them, watching for bristles, which mustn't be touched. For such small parts I use only the right hand, holding it with the left one against trembling.

Finally we can easily embed the separate lobes, and see clearly which of the bristles on the dorsal lobe are actually bristles and which ones are bunches of thin hairs.